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Medical Chemistry and Biochemistry

Exocrine Pancreatic Function Test 13C-Mixed Triglyceride Breath Test

Author: Petr Kocna, Zdislava Vaníčková, Tomáš Krechler, Milan Lukáš, Josef Doseděl, Pavel Kohout

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Chronic pancreatitis could be well diagnosed by histopathology, but for clinical purposes, differential diagnostics and patient follow-up we use mainly imaging procedures and non-invasive pancreatic function tests, if available. In this study we report the 4 years experiences with noninvasive test of exocrine pancreatic function - breath test with 13C-mixed triglycerides (MTG-BT). libators, the mean difference was 4.9% (Pearson

The Role of IgA and IgG Classes of anti-tissue Transglutaminase in Coeliac Screening

Author: Petr Kocna, Zdislava Vaníčková, Miloš Dvořák, Jindřiška Perušičová

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In this study we compared different algorithms using four serology markers of CD in a group of 1451 patients. The correlation of two ELISA methods (Genesis, DPC Millenia) of atTG IgA class was high (r = 0.930), correlations of at TG (DPC) and antigliadin (AG) antibodies were significantly higher in the IgA class than IgG class, Spearman’s coeff. were 0.607 and 0.423 respectively. The clinical benefit of IgG atTG antibodies determination could be exhibited by 7 cases highly positive in IgG class but negative in IgA class of atTG, or by 5 cases in the screening strategy positive with IgG atTG from 78 cases negative in all IgA class antibodies (AGA, EmA, atTG). 

Clinical and Laboratory Aspects of 13C-Breath Test Evaluated by NDIRS Infrared Spectrometry

Author: Petr Kocna, Zdislava Vaníčková, Jan Chleboun

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This study summarizes pre-and post-analytical aspects of the 13C-breath-test(BT) evaluated by means of isotope selective nondispersive infrared spectrometry(NDIRS) -Isomax 4000 (Isodiagnostika). Moreover, the sources of inaccuracy in test results are identified: (a) uncertain baseline 13C abundance, (b) inaccuracy of the spectrometer, and (c) uncertainty in CO2production, which also burden cummulative BT where IR/IRMS measuring instruments are used. 

Hypertext Encyclopedia of Laboratory Methods on the web and CD-ROM

Author: Petr Kocna

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Textbooks, lectures and other educational textsprepared in last years are on-line (mostly in Czech language) available on WWW server of 1st Faculty of Medicine of the Charles University in Prague on URL: http://www.lf1.cuni.cz/ukb/.This presentaion is concerned to our last project of hypertext, multimedia encyclopedia of laboratory methods -GastroLab(URL: http://gelab.zde.cz).This pilot chapter is focused to gastroenterology. There are 47 laboratory methods, described in concise text, supplemented with 38 PowerPoint images, 237 on-line linksto last scientific papers (MEDLINE database) and direct links into National Registry of Laboratory Methods (NCLP = National Reference Table of Laboratory Items) on the server of Ministry of Health of the Czech Republic -URL: http://www.mzcr.cz.The GastroLab is now included into another hypertext project -Encyclopedia of Laboratory Medicine. This CD-ROM publication was issued in December 2002, 2nd updated edition will be ready in June 2003, edited by prof. A. Jabor at co-workers (Institute of Postgraduate Education).

Introduction to Biological Psychiatry

Author: Zdeněk Fišar

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Biological psychiatry occupies itself by mental disorders from biological, chemical and physical point of view. It is postulated that changes in brain signal transmission are essential in development of mental disorders. The purpose of this teaching material is to describe cellular and molecular properties of neurons, mechanisms of psychotropic drugs action and biological basis of mental disorders. Familiarity with biochemistry and physiology is supposed.